Read up to 6 wavelengths in endpoint or kinetic mode for 96- and 384-well plates, and perform spectral scans to determine. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The results of ELISA optical density (OD) values in duplicates were averaged for the following analyses. 4) was followed according to Vanacker et al. 30. 304 signal level saturation. Call 1-800-745-2710. EX range: 340/80 nm EM range: 616/10 nm High fg levels of Streptavidin High fg levels of Streptavidin 2 AlphaScreen. MMP‑1 inhibition assay. Percent radical scavenging activity was determined by comparison with a DMSO containing control (shown in Table 3). ), which was proportional to the number of damaged cells. chen@case. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Money Saved: $340. Created Date: 20051213090753Z measured using a SpectraMax 340 multiwell plate. The. Data are expressed as percent of maximum binding for each protein. 0 1. Binding affinity for denosumab. A standard curve was constructed by sequential dilution of a TNFα standard from 2000-15 pg/mL. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. 6. WST regents of 10% were added to each well, and absorbance was determined at 450 nm using an ELISA reader (SpectraMax® 340, Molecular Device Co. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. The SpectraMax® Gemini™ EM Microplate Spectrofluorometer from Molecular Devices® provides a flexible environment to determine the optimal excitation and emission settings for most fluorescent intensity assays. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Optical density was determined at 490 nm (background values measured at 690 nm were subtracted) using a SpectraMax 340 microplate reader (Molecular Devices, Sunnyvale, CA, USA). ISM Equipment Classification (Group 1, Class A) This equipment is designated as. Briefly, cells were seeded into a. Finally, the absorbance at 450 nM was taken using a SpectraMax ® 340 ELISA plate reader (Molecular Devices, USA). 2 were considered negative for IgM antibody. 315 can't find zero order. In vivo anti-malarial assay in mice. Subsequently, the mixture was heated at 100 °C for 10. Manual: SoftMaxPro User Guide Manufacturer: Molecular Devices Location: SOM-E536. Manufacturer: Molecular Devices Model: SpectraMax The SpectraMax® 340 PC 384 Absorbance Microplate Reader from Molecular Devices provides the necessary tools for absorbance measurement in the visible range. , Sunnyvale CA). Percent viability was calculated by comparison with the control group. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that. 0200-7027. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes. 2. SpectraMax® Microplate Readers and SoftMax® Pro Software. The concentration of Alizarin Red S staining in the samples was determined by comparing the absorbance values with those. The SpectraMax ABS readers use a grating monochromator to select the exact wavelength needed for every assay. Customer Transformation Hub. The increase in fluorescence was determined for 20 min by excitation at 520 nm and emission at 545 nm in a. 04. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. 30. The ability of PTK787/ZK 222584 to inhibit angiogenesis was tested in an in vitro model of capillary sprout formation (64) . Buffer (100 mmol L –1acetate buffer, pH 5. D. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. A microplate reader asThe absorbance was measured at 425 nm and read every 10 min using a Spectramax 340 plate reader (Molecular Devices ®, USA). Liquid handling for random screening was carried out with a Biomek FX from Beckman Coulter (Fullerton, CA, USA) and HYTRA. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. A 340 μL of 50 mM borate buffer (pH 9), 240 μL methanol, 10 μL standard solution or sample, and 20 μL of 0. g. The relative LDH release was determined as the percentage ratio of the optical densities in the media (containing CNMs at each. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. When reading optical density at wavelengths below 340 nm, special UVtransparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Assay setup for a 384-well low-volume plate. Capable of detecting DNA down to 25 ng per well, this model gives users the same sensitivity as conventional systems to produce accurate nucleic acid measurements. SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 5 mm (bottom read) Beam diverges to focal spot near bottom of well. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Oxidative stress induction by H 2 O. Microplate Spectrophotometers. (1988) and recorded at 412 nm. 1: SpectraMax Plus 384 components. SpectraMax 340 (Obsolete), SpectraMax 340PC 384 (Obsolete), Gemini EM reader, SpectraMax 250 (Obsolete) What is h the bandwidth for SpectraMax® plate readers and how do we determine it? The instrument bandwidth for the reader is expressed as half-bandwidth, meaning the full. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. CD154 detection in equine PBMC by an anti-human CD154 antibody (clone 5C8) was confirmed by Western blots and then applied for flow cytometry. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The SPECTRAmax 340PC can mea- TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytes acrossawiderangeofconcentrations. The absorbance was measured at 550 nm with a Spectramax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA). Briefly, cells were sequentially treated with terminal deoxynucleotidyl. Fibrin formation was measured by turbidity at 405 nm using a SpectraMax 340 PC plate reader. After treatment, total cellular RNA was extracted with a cold RNA extraction solution (Ultraspec RNA;Call 1-800-745-2710. Driessen*,MaaikeA. Call 1-800-745-2710. using SpectraMax i3 (Molecular devices, San Jose, USA). The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 3 1. The absorbance values were measured at 450 nm using a SpectraMax® 340 plate reader (Molecular Devices, Sunnyvale, CA, USA). Apoptosis was also measured by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) assay using the TACS Blue label kit (4811-30-K, R&D Systems). 0% and ± 0. Values were determined according to a standard curve (log/log curve-fit). 0 to 4. , Sunnyvale, CA). 314 low lamp level warning. The reaction mixture was incubated for 5 min. Description. 2 Chemical Reagents 1. Table 1. Introduction. Manager: Yinghua Chen, PhD Contact Email: yinghua. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. MCE has not independently confirmed the accuracy of these methods. The reaction was then stopped by adding 50 µL of 1 M sulfuric acid to each well. The absorbance is determined at 620 nm on a SpectraMax 340 PC 384. The absorbance at 540 nm was determined in a spectrophotometer (SPECTRAmax 340, Molecular Devices). Assay buffer was 50 m M Hepes, pH 7. SpectraMaxABS Plus,SpectraMaxM2,SpectraMaxM2e,SpectraMax M3,SpectraMaxM4,SpectraMaxM5,SpectraMaxM5e,SpectraMaxPlus 384 0200-7200 Multi-Mode ValidationPlate. 05 ng/mL; progesterone: 2. 340 nm / 80 nm. Turn on the Spectramax 340PC spectrophotometer using the switch located next to the plug in the back on the right hand side (as you face the spec). Microplate Spectrophotometer Operator’s Manual. The manufacturer of the SpectraMax 340PC Microplate Spectrophotometer is Molecular Devices. Rapid, accurate DNA, RNA, and protein quantitation in a one-touch, full-spectrum micro-volume absorbance reader. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. Samples with OD values of less than 0. The SPECTRAmax 340PC can mea-The extracted stain was then transferred to a 96-well plate, and the absorbance at 562 nm was measured using a SpectraMax 340 plate reader/spectrophotometer(Molecular Devices Corp. When you do a read at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. The SPECTRAmax 340PC can measure. MCE has not independently confirmed the accuracy of these methods. View the Molecular Devices website. 5. The optical density of each well was determined immediately using a microplate reader (SpectraMax-340, Molecular Devices) set to 450 nm. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). 190–850 nm <± 1. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. They are for reference only. Other technologies such as imaging. Service Contracts, Preventative Maintenance and Performance. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. 4- to 5-weeks old female BALB/c mice were purchased from Charles River Laboratories (QC, Canada). All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). SpectraMax Multi-Mode Microplate Readers. TheSpectraMax®Plus384addstheabilitytoread SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Absorbance was then measured at 540 nm (isosbestic point of cytochrome c) and 550 nm (SpectraMax 340; Molecular Devices, Sunnyvale, CA, USA). The assay was carried out in a 96-well plate in a final volume of 100 µl containing 50 mmol/l Tris-HCl, pH 7. Spectral Range. Absorbance measurements were recorded using a SpectraMax 340 (Molecular Devices) at 490 nm on the indicated days (Cell Proliferation Kit I; Roche). Exhibit with us {{>currencyLabel}} Back. The inhibition percentage was determined by linear regression through ranges of 20 to 30. SpectraMax ® Plus 384 optics. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Clotting times (mean ± SD) for rSimukunin and rSV. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. 1 0 100 80 60 40 20 0 4. Quick View. Each experiment was performed in duplicate. reader (SPECTRAmax™340; Molecular Devices, Palo Alto, CA, USA). Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays. #ABS PLUS) SpectraMax® Plus 384 Microplate Reader (Molecular Devices cat. the SpectraMax iD3 is a fantastic plate reader for a wide range of assays. One plate carrier adapter is provided with the instrument. (1993). Emission. 1% PEG 8000 at 37°C. Scanning was done at 600 nm, using a Molecular Devices SpectraMax 340 plate reader. Absorbance plate reader, temperature control, robotics-compatible, 384 well plates, 340-850 nm. Proteolytic activity was determined using the synthetic chromogenic substrate Nα-benzoyl-DL-arginine p-nitroanilide (DL-BApNA), in 96-well plates, according to Ponce-Soto et al. Details. , Sunnyvale, CA) after incubation of solution mixture at 37 °C for 2 h 30 min in the dark. 5), L-ascorbic acid (25. Media in plates were withdrawn by pipetting and 200 μl of DMSO was added to each well to solubilize the formazan crystals. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideHigh-throughput assays for promiscuous inhibitors Brian Y Feng1,3, Anang Shelat1,3, Thompson N Doman2,RKipGuy1 & Brian K Shoichet1 High-throughput screening (HTS) searches large libraries of chemical compounds. The SpectraMax ABS Plus, can accommodate standard 96-well plates and 384-well plates. 5 mL of 1 M acetate buffer (pH 4. The plates were incubated for 4 additional hours at 37°C. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. Cell Assay Cells (5 × 10 5 per well) are plated in triplicate in 96-well plates in 0. Changes in OD 405 nm were followed on the SpectraMAX 340 plate reader. Emission. 05% at 340 nm Photometric stabilization Instantaneous Photometric drift None—continuous referencing of monochromatic output Calibration Automatic before first kinetic read and. Our absorbance plate readers feature our PathCheck Sensor technology and. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise results. One plate carrier adapter is provided with the instrument. automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA) microtiter plate reader. According to LDH assay, a 60 μL of reaction mixture containing catalyst (diaphorase/NAD+), dye solution of iodotetrazolium chloride and sodium lactate was. 7. 5% (w/v) sodium dodecyl sulfate-polyacrylamide gels, and transferred to immobilon polyvinylidenedifluoride membranes (Millipore, Bedford, USA). The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Tunable for filterless flexibility. Not all manufacturers' microplates are the same with regard to design, materials, orIn a typical 100-μL assay with mixtures containing 50 mM MOPS, pH 6. Bacterial growth was measured after every 45 min by recording the absorbance at 595 nm using a SpectraMax 340 spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). The cell growth was determined by measuring the optical density at 600 nm using a spectrophotometer (SpectraMax 340, Molecular Devices, USA). application or budget. 0 3. SpectraMax Multi-Mode Microplate Readers. SpectraMax® Microplate Readers and SoftMax® Pro Software. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. #W50-640) SpectraMax i3 or i3x Multi-Mode Microplate Reader (Molecular Devices; other SpectraMax microplate readers with fluorescence detection are also suitable) SoftMax Pro Software (Molecular Devices) Methods VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. Lightgreenglass,2 mmthick(340 nm) 9:Yellowglass,3 mmthick(405 nm) 10:Darkredglass,3 mmthick(650 nm)automated SpectraMAX 340 microtiter plate reader. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. SoftMax Pro. This Molecular Devices SPECTRAmax 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Pharmacokinetic and pharmacodynamic analysis. Read one sample or up to 384. Immunofluorescence and Aggregation Analysis. The SpectraMax M2 is a dual-monochromator, multi-detection microplate reader with a dual-mode cuvette port and 96 or 384 microplate reading capability. Bertin Precellys Evolution Touch Homogenizer. An absorbance was read at 490/630 nm using a spectraMAX 340 microtiter plate reader. Like CD3, the CD95 death receptor requires binding of immobilized or multimeric antibodies to be activated effectively. These robust readers have been placed in labs from Antarctica to the. SpectraMax Multi-Mode Microplate Readers. In order to detect the Fc-tagged fusion proteins, goat anti-human IgG (Fc Fragment specific; Jackson ImmunoResearch Laboratories Inc, West Grove, PA, USA; Code Number 109-006-098;. SPECTRAmax® 190. Near bottom. 340 nm / 70 nm. Furthermore, the stain was extracted using 10% acetic acid, and its absorbance was measured at 595 nm in a microtiter plate reader (SpectraMax 340; Molecular Devices). Example: [Drawer]. The maximum specific growth rate μ was calculated from the exponential growth phase ( 52 ) and plotted against the concentration of the different drugs. The SpectraMax 340PC 384 and SpectraMax Plus 384 read both 96-and 384-well microplates. 06 ng/mL; FSH: 0. Data were expressed as the mean LPS-treated cells (100%) vs. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. The SpectraMax ABS Plus reader was heated to 37ºC prior to setting up the. Although all samples initially were analyzed at the same volume, the volume of sample. Developed plates were read on MSD reader Sector Imager S 600, Model 1200, with Discovery Workbench Software (version 4. Page 1 ® SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0049 Rev. 1 mL of culture media with 10% FBS. Created Date: 20051213090753Zmeasured using a SpectraMax 340 multiwell plate. Upgradeable modules are also available including western blot, cell imaging, and fast. In this study we used tetanus toxoid (TT) as a well-defined antigen to. The Molecular Devices SpectraMax 340PC384 Microplate Reader can run both standard spectrophotometer and microplate reader applications on the same instrument. For more sample throughput, the system can The optical absorption was measured at 450 nm for BUN and 490 nm for creatinine with a spectrometer (SpectraMax 340). After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. Other technologies such as imaging. Absorbance values were read at 570 nm on an automated SpectraMAX 340 (Molecular Devices, Sunnyvale, CA, USA). The ratio of red to green fluorescence intensity was calculated. Triton X-100 (see Note 2). 00 USD. 2. The plates were read using an ELISA microplate reader (Molecular Devices SPECTRAMAX 340 PC 384, Sunnyvale, California, USA) at 450 nm. Recalcification (clotting) time was determined by monitoring absorbance at 650 nm at 10-sec intervals in a SpectraMax 340 microtiter plate reader, with onset time (the time to a linear increase in the OD, which reflects the maximal rate of formation of insoluble fibrin) set at an OD of 0. Molecular Devices SpectraMAX 340 Microplate Reader. edu Contact Phone: 216-368-8962. All variables were tested in three independent cultures for each experiment, which was repeated twice (n = 6). The contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, which makes it possible to perform kinetic analysis of solid- pathways and protein activation and expression in one system. 5 stars - 1602 reviewsThis assay typically develops in 1. 0, MSD) or Spectramax 340 (Molecular Devices). TDP-43 stress granular cells were treated with 70% ice-cold. Then the samples were incubated for 1 h with human IL6 detecting antibody and then with HRP conjugate for 40 min. Microplate Readers. 1 All Necessary Cables and Cords Manuals General Specifications Dimensions (in. Readings were made in a microplate spectrophotometer (SpectraMAX 340, Molecular Devices, Sunnyvale, California, USA) with wavelength set to 450 nm and correction set. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer to the Obsolete Microplate Reader Models article for additional information. Screening for Antigen-Specific IgG Producing Clones by Capture ELISA. Near bottom. Background: Ketamine can act as a multifunctional neuroprotective agent by inhibiting oxidative stress, cellular dysfunction, and apoptosis. Data were expressed as the mean percent viable cell vs. 5 mL of 1 M acetate buffer (pH 4. 314 low lamp level warning. Briefly, cells were seeded into a. Plate reader (SpectraMax 340 PC plate reader or comparable plate reader). AoSMCs were seeded into 6-well culture plates. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. 303 unable to cal dark current. Each SpectraMax® 340 PC 384 Microplate Reader has an optical system built around a monochromator. Filterless Absorbance Microplate Readers provides fast & consistent results with steady temperature enabling a wide range of assays - ELISA reader, microbial growth, & protein quantitation. 0. 306 plate air cal. 306 plate air cal fail,low light. SpectraMAX 340. Optical density was measured at 570 nm using a microplate reader (SpectraMAX 340, Molecular Devices Co. Microscopy. See More Microplate Reader / Microtiter Plate Reader. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. The SpectraMax 340PC384 Microplate reader supports various absorbance assay categories, such as protein quantitation, endotoxin detection, microbial growth, ELISAs. Or scan up to 384 wells at any time to determine the best assay conditions. 4. The ABS reader ranges from. Or scan up to 384 wells at any time to determine the best assay conditions. 5% phosphoric acid) in microplates at room temperature for 10 min. Please see photos. Molecular Devices Spectramax 340 Absorbance Plate Reader (1997) Molecular Devices SpectraMax i3x Multi-Mode Microplate Reader (2016) Vernier UV-VIS Spectrophotometer (5) Vernier VIS Spectrophotometer (30) Infrared Spectroscopy. Molecular Devices SpectraMax 340 PC 384 Microplate Reader. Tailored to your different needs, these kits are configured and optimized for Molecular Devices SpectraMax® microplate readers with preconfigured protocols provid- plates can be used in the SPECTRAmax PLUS 384. using SpectraMax i3 (Molecular devices, San Jose, USA). , Fremont, CA, USA). Spectrophotometer (SpectraMax 340). 001/min at 425 nm. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The absorbance was measured at 340 nm for 2 min at a 15 s time interval. Rate of fibrinolysis. 4; 100 m M NaCl, 0. Finally, after another 45 min of decolorization, 100 µL of the solution was transferred to a new well and the amount of crystal-violet stain in the decolorizing solution was measured with a microtiter plate reader (SpectraMAX 340 Adjustable Microplate Reader; Molecular Devices Ltd. 312 gain calibration failed. Description 1. Absorbance at 405 nm (Abs 405) was obtained for both plates before (background) and after 1-h incubation at 37°C using a microplate reader (SpectraMax 340 PC384, Molecular Devices). Nitric Oxide Assay. Measurements of OD at 540 nm were then performed in a microtiter plate reader (SpectraMax 340 microplate reader; Molecular Devices Corp. 2 0. or microplate reader (e. After 24 h, 48 h, 72 h or 96 h, cell viability was measured by CellTiter 96® AQueous One Solution cell proliferation assay (Promega, Madison, WI, USA). The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. This was followed by an additional incubation at 37 °C for 30–60 min. SpectraMax M2, while top and bottom reads are possible on the SpectraMax M2 e. A. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Following HRP-conjugated secondary antibodies, goat anti-rat IgG (Kirkegaard & Perry. Analysis of ALA by Ehrlich’s Reagent Assay. 2. Wavelength Range. The SpectraMax® 190 Absorbance Microplate Reader is the ideal equipment for many life science applications including DNA analysis. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. , Sunnyvale, CA). 1 mM MnCl2 and 25 mM CHES buffer, pH 9. The microtiter plate was then incubated in the dark for 2 h at 37°C. This tunable visible absorbance reader is for 96- and 384-well microplates and acquires data in endpoint, kinetic, and spectral scan modes using wavelengths from 340-850 nm, tunable in 1. 6, 2 mmol/l DTT, 10 mmol/l MnCl 2 and 10 mmol/l pNPP; the PP2Cα enzyme concentration was 400nmol/l. Molecular Devices. Amino acid analysis was performed on a Pico-Tag amino acid analyzer (Wat er System) as. The cytotoxicity of the CDDP loaded CHAMs were done by treating the FaDu cells with DMEM supplemented with 0. TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate spectrophotometersproviderapidandsensitivemeasurementsofavarietyofanalytesacross awiderangeofconcentrations. The SpectraTest validation package provides a NIST-traceable solution for validating optical performance of the SpectraMax Plus 384 reader and does it automatically. SpectraMax 340 (Serial number starting with M) SpectraMax 340PC (Serial number starting with L) SpectraMax Plus (Serial number starting with P) Tmax Also refer. Call 1-800-745-2710. SpectraMax 340PC384 reads 96-well plates and 384-well plates and strip-well plates. When readin g optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. The plate was covered and incubated for one hour at room temperature. PathCheck SensorThe optical density value was measured at absorbance 450 nm by a microplate reader (SpectraMAX 340, molecular devices, USA) for cell viability. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. 1. The activity was measured in a SpectraMAX 340 plate reader at 37°C and 410 nmol/l for 120 sec. The cells were pretreated, serum starved for 24 h, and then treated with NK-cdM priora SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). SpectraMax 190 reads 96-well and strip-well plates. Top and bottom reads are available for fluorescence, ti me-resolved fluorescence and luminescence detection. The cell death was calculated as follows:Absorbance was monitored at 450 nm (SpectraMax 340). 8–250 nM) was measured as follows: the prothrombinase reaction proceeded for 3 min and was stopped by the addition of 10 mM EDTA. Plates were read at a wavelength of 405 nm with a reference wavelength of 620 nm (Spectramax 340 PC). Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. When reading optical density at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the far UV spectra must be used. Wallac 1450 Microbeta TRILUX Liquid Scintillation & Luminescence Counter $1,000. Supplier: Molecular Devices. ). control. The narrow bandwidth used by the model increases measurement accuracy and broad analysis over. LDH release was measured with a commercial kit following the manufacturer’s instructions (LDH Cytotoxicity Detection Kit, TaKaRa). 190-1000 nm, tunable in 1 nm increments. Afterwards, absorbance at 595 nm was measured with spectrophotometer (SpectraMax 340, Molecular Devices, USA). Also for: Spectramax 190, Spectramax 340pc384, Versamax. . Molecular Devices ThermoMax is a high-efficiency, temperature-regulated, 96-well microplate reader. The metals were preincubated with A for 5 min when added before heme. 0 nm increments Wavelength Bandwidth: 2 nm Wavelength Accuracy: < ± 1. The Spectra Catalina 340z produces 14 gallons (53 liters) of water per hour, making it the perfect watermaker for the midsize yacht with two to four people onboard. To. spectramax i3 multi-mode platform, spectramax i3, spectramax i3 system, scanlater western blot system, spectramax minimax imaging cytometer, softmax pro software, metamorph software, spectramax i3 platform. The SpectraMax® iD3 Multi Mode Microplate . After then, 10% of ammonium hydroxide solution was added to neutralize the aqueous solution of the ARS extracts, and the absorbance values were measured using a SpectraMax® 340 plate reader at 405 nm. 8 pin mini DIN (Spectramax 340) to 9D (PC) serial data transfer cable. About The SpectraMax GEMINI EM is controlled by an external computer running SoftMax ® Pro software which provides integrated instrument control, data display, and statistical data analysis. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Analysis of ALA by Ehrlich’s Reagent Assay. When you do reads at wavelengths below 340 nm, you must use special UV-transparent, disposable or quartz plates to allow transmission of the deep UV spectra. Anti-proteolytic activity. Molecular Devices Spectramax 340PC instructions. Wells were washed three times. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deep UV spectra must be used. 5), L-ascorbic acid (25. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Assay setup for a 384-well low-volume plate. Plates coated with no antigen, canine CD19, streptavidin alone, and streptavidin with irrelevant human avitag-CD3ε (Acro Biosystems, Newark, DE) were used as negative controls. Water – distilled water or equivalent. With SOFTmax PRO, the contents of the wells in a microplate can be mixed automatically by shaking before each read cycle, making it possible to. 10× trypsin 0. a SpectraMAX 340VR microplate reader (Molecular Devices, Sunnyvale, CA, USA). LAL Reagent Grade ™ Multi-well Plates (Lonza cat. 303 unable to cal dark current. Not all manufacturers' microplates are the same with regard to design, materials, or76358-624. , Sunnyvale, CA). • TRF-based with 340/80 nm EX and 616/10 nm EM 0200-7027: EX range: 340/80 nm EM range: 616/10 nm: High fg levels of Streptavidin: High. Despite the relevance of adaptive immunity against equine pathogens antigen-specific T cell responses of horses are not well characterized and the lack of insight into T cell responses hampers the understanding of the pathogeneses of important diseases. LBA Formats for Pharmacokinetic AssaysAfter treatment, 10 μl of CCK-8 was added to each well, and the incubation was continued at 37°C for 2 h in the dark. The optical density of each sample was analyzed at 450 nm with a reference reading at 630 nm using a SpectraMax 340 absorbance plate reader (Molecular Devices, Union City, CA). ABS reader ranges from 340-850 nm in wavelength and the ABS Plus reader ranges from 190-1000 nm, which is equivalent to having 510 or 810 free filters, respectively. 7 macrophages were obtained from ATCC (Manassas, VA). Availablility: By Availability Molecular Devices SpectraMAX 340 Microplate Reader. Narrow bandwidths deliver increased measurement accuracy and linearity over a wide range of assays. 315 can't find zero order. SpectraMax Microplate Readers. #25-340) Pyrogen-free Dilution Tubes (Lonza cat. Molecular Devices’ PathCheck® sensor technology. FXII or PK activation by FXIa was tested as follows: FXII or PK (200nM) was incubated with or without FXIa (25nM) in TBS, 0. 00 USD. Western blot. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates and cuvettes that allow transmission of the. Values were determined according to a standard curve (log/log curve-fit). 4. Following incubation, 100 μl of solution was transferred to new wells and the color change in the solution was measured with a microtiter plate reader (SpectraMAX 340 Tunable Microplate Reader; Molecular Devices Ltd. The antibodies originally defining this molecule by inducing apoptosis, anti-Fas and anti-APO-1 , were of the IgM or IgG3 subtype, respectively. The adapter is.